Journal: Vaccines

Article Title: Compatibility and Stability of a Shigella Polysaccharide—Protein Conjugate Antigen Formulated with Aluminum Salt and CpG 1018 ® Adjuvants

doi: 10.3390/vaccines14010010

Figure Lengend Snippet: Binding interactions of aluminum hydroxide (AH) adjuvant with S. sonnei O-PS-IpaB conjugate antigen and the CpG oligonucleotide adjuvant. ( A , B ) Percent adsorption of conjugate antigen to AH at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured using micro-BCA assay. “#” indicates value is equal to or greater than 97% (based on the estimated LOQ of the assay). ( C , D ) Percent adsorption of CpG to AH at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured using UV at 260 nm, where “-” indicates formulations without CpG and “*” indicates formulations where CpG has precipitated (see text). ( E , F ) Zeta potential values of AH at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured by electrophoretic light scattering (ZetaPals). Formulations contained either 2 or 200 mM sodium phosphate buffer in 0.15 M NaCl, 0.05% w/v LDAO, at either pH 7.0 or 5.8 as indicated. Error bars in panels ( A , D ) denote the SD from n = 4 (two vials analyzed at n = 2), panels ( B , E ) denote range from n = 2 (1 vial analyzed at n = 2), and panels ( C , F ) denote range from n = 2 (one vial analyzed at n = 2). AH—Alhydrogel ® and CpG—CpG 1018 ® .

Article Snippet: Zeta potential values of aluminum salt adjuvants in different formulations were measured using a ZetaPALS (Brookhaven Instruments Corporation, Nashua, NH, USA, NanoBrook 90Plus PALS) along with a solvent-resistant electrode (Brookhaven Instruments Corporation, Nashua, NH, USA, BI-SREL, SR-0338) conditioned with 1.5 mL of 0.9% w/v saline solution prepared in deionized water and measured using a cuvette.

Techniques: Binding Assay, Adjuvant, Adsorption, BIA-KA, Zeta Potential Analyzer

Journal: Vaccines

Article Title: Compatibility and Stability of a Shigella Polysaccharide—Protein Conjugate Antigen Formulated with Aluminum Salt and CpG 1018 ® Adjuvants

doi: 10.3390/vaccines14010010

Figure Lengend Snippet: Binding interactions of aluminum phosphate (AP) adjuvant with S. sonnei O-PS-IpaB conjugate antigen and the CpG oligonucleotide adjuvant. ( A , B ) Percent adsorption of conjugate antigen to AP at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured using micro-BCA assay. “#” indicates value is equal to or greater than 97% (based on the estimated LOQ of the assay). ( C , D ) Percent adsorption of CpG to AP at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured using UV at 260 nm, where “-” indicates formulations without CpG and “*” indicates formulations where CpG has precipitated (see text). ( E , F ) Zeta potential values of AP at pH 7.0 and 5.8 in 2 and 200 mM phosphate buffers, respectively, as measured by electrophoretic light scattering (ZetaPals). Formulations contained either 2 or 200 mM sodium phosphate buffer in 0.15 M NaCl, 0.05% w/v LDAO, at either pH 7.0 or 5.8 as indicated. Error bars in panels ( A , D ) denote the SD from n = 4 (two vials analyzed at n = 2), panels ( B , E ) denote range from n = 2 (1 vial analyzed at n = 2), and panels ( C , F ) denote range from n = 2 (one vial analyzed at n = 2). AP—Adju-phos ® and CpG—CpG 1018 ® .

Article Snippet: Zeta potential values of aluminum salt adjuvants in different formulations were measured using a ZetaPALS (Brookhaven Instruments Corporation, Nashua, NH, USA, NanoBrook 90Plus PALS) along with a solvent-resistant electrode (Brookhaven Instruments Corporation, Nashua, NH, USA, BI-SREL, SR-0338) conditioned with 1.5 mL of 0.9% w/v saline solution prepared in deionized water and measured using a cuvette.

Techniques: Binding Assay, Adjuvant, Adsorption, BIA-KA, Zeta Potential Analyzer

Journal: Discover Applied Sciences

Article Title: Exploring marine algae-derived phycocyanin nanoparticles as a safe and effective adjuvant for sunscreen systems

doi: 10.1007/s42452-024-05665-z

Figure Lengend Snippet: Fig. 1 Fabrication and characterization of PCNP. A PC solution (right) and PCNP suspension (left). B Particle size distribution of PCNP. C Transmission electronic microscope (TEM) image of PCNP (indicated with arrows). D Fluorescence emission spectrum of PC (red), and PCNP (gray)

Article Snippet: Dynamic light scattering (DLS) was utilized to determine the size distribution and polydispersity index (PDI) of PCNP nanoparticles (DLS, NanoBrook 90Plus, PALS, Brookhaven Instruments, USA.).

Techniques: Suspension, Transmission Assay, Microscopy, Fluorescence

Journal: Discover Applied Sciences

Article Title: Exploring marine algae-derived phycocyanin nanoparticles as a safe and effective adjuvant for sunscreen systems

doi: 10.1007/s42452-024-05665-z

Figure Lengend Snippet: Fig. 5 UV absorption effects of PC and PCNP gels. A Carbomer gels containing an equal concentration of PC (left) and PCNP (right). B UV absorption of PC and PCNP gels in UVB region (280–320 nm)

Article Snippet: Dynamic light scattering (DLS) was utilized to determine the size distribution and polydispersity index (PDI) of PCNP nanoparticles (DLS, NanoBrook 90Plus, PALS, Brookhaven Instruments, USA.).

Techniques: Concentration Assay

Journal: Discover Applied Sciences

Article Title: Exploring marine algae-derived phycocyanin nanoparticles as a safe and effective adjuvant for sunscreen systems

doi: 10.1007/s42452-024-05665-z

Figure Lengend Snippet: Fig. 6 Results of skin permeation study. A In vitro skin permeability of PCNP (n = 3, all data are presented as the mean ± SD. Significant differ- ence analysis was performed when compared with 2 h group, ***p < 0.001). B In vivo absorption of PCNP gel. DEACCA as a small molecule control, emits green fluorescence, while PCNP inherently exhibits a purplish-red fluorescence, both of which serve to self-trace their loca- tions (indicated with arrows)

Article Snippet: Dynamic light scattering (DLS) was utilized to determine the size distribution and polydispersity index (PDI) of PCNP nanoparticles (DLS, NanoBrook 90Plus, PALS, Brookhaven Instruments, USA.).

Techniques: In Vitro, Permeability, In Vivo, Control, Fluorescence

Journal: Discover Applied Sciences

Article Title: Exploring marine algae-derived phycocyanin nanoparticles as a safe and effective adjuvant for sunscreen systems

doi: 10.1007/s42452-024-05665-z

Figure Lengend Snippet: Fig. 4 UV radiation absorp- tions by PC (A) and PCNP (B) determined by UV spectro- photometer (n = 3, all data are presented as the mean ± SD (n = 5, all data are presented as the mean ± SD. different lowercase letters a, b, c and d represent the significance levels in the post Tukey HSD test))

Article Snippet: Dynamic light scattering (DLS) was utilized to determine the size distribution and polydispersity index (PDI) of PCNP nanoparticles (DLS, NanoBrook 90Plus, PALS, Brookhaven Instruments, USA.).

Techniques: